REPLICATION OF TYPE-2 HERPES-SIMPLEX VIRUS IN HUMAN ENDOCERVICAL TISSUE IN ORGAN-CULTURE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 57  (4) , 460-471
Abstract
The replication of type 2 herpes simplex virus in human endocervical tissue in organ culture was investigated. The temporal profile of virus replication was related to the initial virus inoculum; high input inocula induced a rapid increase in virus titer while lower multiplicities induced a more slow-rising increase in virus titer. Explants were apparently capable of initiating and supporting virus replication for at least 2 wk following establishment of the culture. Virus yields were optimal when explants were cultured at 37.degree. and in serum-supplemented medium. Explants also supported the replication of type 1 herpes simplex virus and a non-human herpes simplex virus (pseudorabies virus). The optimal conditions for replication of type 2 herpes simplex virus in human endocervical explants were established and will provide a model permitting precise investigation of lytic or other virus-cervical cell interactions, and their possible relationship to herpes virus-induced pre-invasive carcinoma of this organ.