A kinetic study of the assimilation of 15N-labelled nitrate in rice seedlings

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Abstract
A 15N kinetic-analysis of the assimilation of nitrate nitrogen in the roots of rice seedlings indicated that (1) nitrate was rapidly reduced to ammonia in the roots, where it was incorporated into glutamine and glutamic acids; (2) the pattern of nitrate assimilation into amino acids was very similar to that of ammonium assimilation; and (3) the pattern of nitrogen incorporation into protein was also similar to that of the incorporation with NH4-feeding. In the shoots, alanine, serine, glutamic acid, γ-amino butyric acid and aspartic acid were relatively strongly labelled with 15N as compared with the other amino acids. A different mechanism of nitrogen assimilation seems to operate in between the photosynthetic and non-photosynthetic organs of the plants.

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