CULTURE OF RAT SERTOLI CELLS ISOLATED WITH A MODIFIED PROCEDURE - MORPHOLOGICAL IDENTIFICATION OF CELL-POPULATION AND CELL REACTIVITY

  • 1 January 1981
    • journal article
    • research article
    • Vol. 19  (3) , 135-+
Abstract
The effects of growing Sertoli cells isolated from rat seminiferous epithelium by a modified procedure, and responses of these cells to dB[dibutyryl]cAMP or FSH stimulation were estimated using morphological methods. The modified isolation procedure included repeated mechanical rinsing of tubule pieces with a modified EDTA containing Hanks medium. Streptodornase instead of DNase was added to the trypsin containing medium, resulting in a better dispersion of tubule components. The culture conditions remained unmodified. A high degree of homogeneity of the cultured cell population and an evident reactivity of these cells to dBcAMP and FSH were achieved. Giant cells, visible in monolayer among the typical Sertoli cells, were observed. Contrary to a small number of myoid cells, surviving in the culture, these giant cells did not show a positive reaction to alkaline phosphatase.

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