The electrophoretic analysis of normal human serum

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Abstract
By the use of the Lamm scale method with the new Tiselius electrophoresis apparatus, a quantitative analysis of the protein components of normal human serum was made. The mean values found from several sera were albumin 59%, [alpha]. [beta], and [gamma], globulins 4.5, 11.0 and 25.5% respectively. The amounts appear to be independent of pH and conc. Ultracentrifugal analysis of the same sera gave albumin 78%, total globulin 22%, values which show considerable divergence from the electro-phoretic data. Certain possible modifying influences are discussed, in relation to the amts. of the components.

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