Mutations in anionic trypsinogen gene are not associated with tropical calcific pancreatitis

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Abstract
Of the nine members of the human trypsinogen gene family, only PRSS1, PRSS2, and PRSS3 are functional genes coding for cationic, anionic, and meso-trypsinogen isoforms, respectively. The anionic form accounts for about one third of the total trypsins in pancreatic juice. We investigated whether mutations in the anionic trypsinogen gene may contribute to the pathogenesis of tropical calcific pancreatitis. Of 20 mutations reported to date in the PRSS1 gene, 17 are clustered in exons 2 and 3 only. The remaining three are in the promoter region but reported in isolated patients. Hence we initially screened exons 2 and 3 of the anionic trypsinogen gene in 68 well characterised Indian patients with tropical calcific pancreatitis.6 Subsequently, we also sequenced the promoter, complete coding region, and the flanking region in an attempt to look for any novel mutation.