Immunochemical Relationship between Soluble and Insoluble Lens Proteins

Publisher Website
Google Scholar
Abstract
The relationship between soluble and insoluble proteins in the bovine, human and dogfish lens has been studied by immuno-electrophoresis and urea starch gel electrophoresis at pH 3.0. The result from the immuno-electrophoresis experiment indicated that insoluble protein derived from bovine and human lens is composed of α- and β-crystallins while that obtained from dogfish lens contains γ- and β-crystallins. The urea starch gel electrophoretic patterns of α-crystallin and insouble protein in bovine and human lens showed considerable similarity, however, those of dogfish γ-crystallin and dogfish insoluble protein showed significant differences in their mobilities. The cross-reaction between bovine α-crystallin and bovine insoluble suspension was studied by the quantitative complement fixation test, using rabbit antisera to bovine α-crystallin. The results indicated that 5 to 10% of the total insoluble protein has α-crystallin in a form available for the reaction with the antibody. Similar results were observed when dogfish γ-crystalin and dogfish insoluble suspension was studied.

This publication has 0 references indexed in Scilit: