Kinetic Determination of Trypsin with an Ammonia Gas-Sensing Membrane Electrode
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 15 (8) , 709-719
- https://doi.org/10.1080/00032718208069504
Abstract
Trypsin activity is monitored in a simple kinetic method with an ammonia gas sensor. The method is based upon the hydrolysis of .alpha.-benzoyl-arginine amide resulting in the production of ammonia. Day-to-day precision range of .+-. 1.9% to 4.4% and a within-run precision range of .+-. 1.8% to 4.2% were measured for the working range of 0.5-20 .mu.g/ml-1 trypsin. An average of 97 .+-. 3.4% added trypsin was recovered from rat pancreatic juice in recovery experiments.This publication has 6 references indexed in Scilit:
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- A spectrophotometric determination of trypsin and chymotrypsinBiochimica et Biophysica Acta, 1955
- The estimation of tryptic activity in duodenal contentsBiochemical Journal, 1953
- Estimation of Trypsin in Duodenal JuiceBMJ, 1952