GALACTOSIDASE IMMUNOSORBENT TEST FOR CARCINOEMBRYONIC ANTIGEN

  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (8) , 2845-2849
Abstract
A galactosidase immunosorbent test for carcinoembryonic antigen (CEA) is described in which the amount of galactosidase adsorbed to a cellulose disc is a hyperbolic function of CEA concentration. Molecules with CEA-like activity can be characterized by mathematical analysis of data obtained from the galactosidase immunosorbent test. By such analysis CEA-reactive molecules in normal human plasma were distinguished from normal cross reacting antigen and authentic CEA. Variation of the amount of antibody-enzyme conjugate used in the galactosidase immunosorbent test permitted CEA-reactive material in plasma of a patient with rectal carcinoma to be antigenically distinguished from the CEA-reactive material in urine of a patient with bladder carcinoma. The galactosidase immunosorbent test is a useful tool for analysis of CEA reactive molecules.

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