LATENT BETA-TRANSFORMING GROWTH-FACTOR IN NONTRANSFORMED AND KIRSTEN SARCOMA VIRUS-TRANSFORMED NORMAL RAT-KIDNEY CELLS, CLONE 49F
- 1 January 1984
- journal article
- research article
- Vol. 44 (12) , 5538-5543
Abstract
Normal rat kidney cells of the clone 49F and their Ki-MSV[Kirsten sarcoma virus]-transformed counterparts spontaneously release the same transforming growth factor (TGF) activity in an inactive form. By acidification followed by neutralization prior to assay, this TGF activity is unmasked and promotes anchorage-independent growth of the NRK-49F indicator cells in the presence of epidermal growth factor. The TGF activity released by both cell types has an apparent MW of 9000 under acidic conditions, does not compete for binding to epidermal growth factor receptors, is heat resistant but dithiothreitol and trypsin sensitive and therefore is of the .beta.-TGF class.This publication has 2 references indexed in Scilit:
- Mouse embryos contain polypeptide growth factor(s) capable of inducing a reversible neoplastic phenotype in nontransformed cells in cultureJournal of Cellular Physiology, 1982
- Transforming growth factor(s) production enables cells to grow in the absence of serum: an autocrine system.Proceedings of the National Academy of Sciences, 1982