A MODIFIED METHOD FOR THE INVIVO LABELING OF RED-BLOOD-CELLS WITH TC-99M - CONCISE COMMUNICATION

  • 1 January 1982
    • journal article
    • research article
    • Vol. 23  (4) , 315-318
Abstract
The rate of incorporation of 99mTc into red blood cells pretinned in vivo was measured by collecting blood samples in stannous DTPA [diethylene triamino pentaacetic acid] solution, which served as a competing ligand for 99mTc. This collection technique permitted a measurement of high-affinity red-cell labeling efficiency at the instant of sampling. At 0.5 min after injection only 62% of Tc is tightly bound to the red cell; this rises to 94.5% at 10 min. Based on the graded labeling of the red cells, the in vivo labeling procedure was modified by isolating pertechnetate and red blood cells tinned in vivo in a syringe during the first 10 min of labeling. The pertechnetate is thus prevented from distributing to extravascular compartments, and 90% of the injected 99mTc is firmly bound to red blood cells at the time of injection. In a series of 23 patients, 7 were tested with the in vivo method and 7 with the modified in vivo method, and 9 patients were tested with each method on separate occasions. A decrease in gastric activity and improved image quality were found with the modified method compared with the standard method of in vivo red cell labeling.

This publication has 0 references indexed in Scilit: