EFFECT OF ESTROGEN AND ANDROGEN UPON UPTAKE OF GLYCINE-C14BY RAT UTERI IN VITRO: LACK OF CONCENTRATION DEPENDENCE1

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Abstract
IN A recent report (1) dealing with the effect of testosterone propionate (T.P.) upon incorporation of labeled glycine into mouse kidney slices, data were presented showing that the effect of T.P. appeared to depend upon the concentration of glycine in the incubation medium. Thus, treatment of female A/Jax mice with 1 mg. T.P./day for 2 days resulted in an apparent increase of 35–70% in incorporation rate when the concentration of glycine in the medium was 1.0 mM or lower; when the concentration of the latter was increased to 5 mM or more, the difference between incorporation rates for control and treated animals was less than 20%. These facts were interpreted as indicating that T.P. acts, in part at least, by facilitating the intracellular accumulation of glycine. The incorporation of radio-glycine into proteins of surviving segments of rat uteri has been shown to be substantially increased by the administration of testosterone (2) as well as estradiol (2, 3). Simultaneous administration of both hormones effected an increase greater than either alone (2), leading to the suggestion that different mechanisms were involved in each case.