Preclinical Diagnosis of African Swine Fever in Contact-Exposed Swine by a Real-Time PCR Assay
Open Access
- 1 January 2005
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 43 (1) , 112-119
- https://doi.org/10.1128/jcm.43.1.112-119.2005
Abstract
A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.Keywords
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