Serum Alkaline Phosphatase: Total Activity and Isoenzyme Determinations Made by Use of the Centrifugal Fast Analyzer

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Abstract
We present a kinetic method in which the centrifugal analyzer is used to determine total alkaline phosphatase activity and to measure the isoenzymes in bone, liver, and the L-phenylalanine-sensitive fraction (intestine, tumor, and placenta). Measurements were made at 30°C in diethanolamine buffer, with p-nitrophenylphosphate as substrate. The alkaline phosphatase isoenzymes were separated and measured by selective chemical inhibition with 10 mmol/liter L-phenylalanine and 3.3 mol urea per liter. Analyses were performed on sera of a group of healthy pediatric and young adult volunteers and, in addition, on sera from patients with clinically documented osteoblastic disorders and hepatobiliary diseases. The precision and accuracy of the centrifugal analyzer were evaluated, as was the suitability of the instrument for routine clinical laboratory use.

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