Site-specific methylation of 16S rRNA caused by pct, a pactamycin resistance determinant from the producing organism, Streptomyces pactum
- 1 November 1991
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 173 (22) , 7213-7218
- https://doi.org/10.1128/jb.173.22.7213-7218.1991
Abstract
Ribosomal resistance to pactamycin in clones of Streptomyces lividans containing DNA (pct) from Streptomyces pactum, the pactamycin producer, involves methylation of 16S RNA. The modified residue A-941 in S. lividans 16S rRNA (A-964 in the homologous Escherichia coli sequence) is converted to 1-methyladenosine, and the ribosomal ability to bind pactamycin is reduced or abolished.Keywords
This publication has 25 references indexed in Scilit:
- Binding sites of the antibiotics pactamycin and celesticetin on ribosomal RNAsBiochimie, 1991
- Binding of tRNA to the ribosomal A and P sites protects two distinct sets of nucleotides in 16 S rRNAJournal of Molecular Biology, 1990
- Use of a Fractionated, Coupled Transcription-Translation System in the Study of Ribosomal Resistance Mechanisms in Antibiotic-producing StreptomycesMicrobiology, 1989
- Model for the three-dimensional folding of 16 S ribosomal RNAJournal of Molecular Biology, 1988
- The emerging three-dimensional structure and function of 16S ribosomal RNABiochemistry, 1988
- A detailed model of the three-dimensional structure of Escherichia coli 16 S ribosomal RNA in situ in the 30 S subunitJournal of Molecular Biology, 1988
- Sites of action of two ribosomal RNA methylases responsible for resistance to aminoglycosidesJournal of Molecular Biology, 1987
- Pactamycin binding site on archaebacterial and eukaryotic ribosomesBiochemistry, 1987
- Photoaffinity labeling of the pactamycin binding site on eubacterial ribosomesBiochemistry, 1985
- Cloning and Expression of the Tyrosinase Gene from Streptomyces antibioticus in Streptomyces lividansMicrobiology, 1983