Determination of sulphide in blood with an ion-selective electrode by pre-concentration of trapped sulphide in sodium hydroxide solution
- 1 January 1988
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 113 (5) , 839-840
- https://doi.org/10.1039/an9881300839
Abstract
A method for the determination of sulphide in blood using an ion-selective electrode and a wash-bottle reactor has been developed. The method involves liberation of blood sulphide by addition of acid and subsequent trapping of the liberated H2S gas in NaOH solution. The H2S is determined directly with the ion-selective electrode and without any interference from other blood components. This method is ten times more sensitive than the conventional Conway microdiffusion determination and a sulphide detection limit of approximately 3 × 10–7 M(10 µg l–1) can be achieved. Blood sulphide concentrations in 12 non-fatal cases of acute hydrogen sulphide poisoning ranged from 1.3 × 10–6 to 18.8 × 10–6 M(40–600 µg l–1).Keywords
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