Isoflavone Characterization and Antioxidant Activity of Ohio Soybeans

Publisher Website
Google Scholar
Abstract
Seventeen Ohio soybeans were screened for isoflavone content and antioxidant activity. Isoflavone content was determined by C18 reversed phase high-performance liquid chromatography coupled with a photodiode array detector. Antioxidant activities of soybean extracts were measured using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical and photochemiluminescence (PCL) methods. The highest and lowest total isoflavone contents were 11.75 and 4.20 μmol/g soy, respectively, while the average was 7.12 μmol/g soy. Antioxidant activities of soybean extracts ranged from 7.51 to 12.18 μmol butylated hydroxytoluene (BHT) equivalent/g soy using the DPPH method. Lipid and water soluble antioxidant activities of soybean extracts ranged from 2.40 to 4.44 μmol Trolox equivalent/g soy and from 174.24 to 430.86 μmol ascorbic acid equivalent/g soy, respectively, using the PCL method. Keywords: Soybean cultivars; isoflavones; antioxidant activities