Chromatin structure of active and inactive human X-linked phosphoglycerate kinase gene
- 1 January 1986
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 12 (1) , 73-80
- https://doi.org/10.1007/bf01560729
Abstract
We have carried out a comparative analysis of DNase I sensitivity of the active and inactive X-linked phosphoglycerate kinase (Pgk)genes in human lymphoblast and fibroblast cultured cells. Three DNase I-sensitive regions were detected: a 5′ hypersensitive site, a sensitive region in the interior of the gene and a 3′ slightly sensitive site which we previously reported and have now mapped with some precision. A comparison of these sensitive sites in single and multiple X cell lines indicates that the sensitive sites are unique to the active X chromosome. A similar study of an X-linked Pgkpseudogene shows no difference in DNase I sensitivity between the pseudogenes on the active and inactive X chromosomes. These latter results imply that sex chromatin does not confer a unique level of DNase I resistance to DNA on the inactive X chromosome. The exact role of sex chromatin in differential DNase I sensitivity of genes on the inactive and active X chromosomes is discussed.This publication has 20 references indexed in Scilit:
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