Enzyme immunoassay of estrogen-like substances in plasma, with polyethylene glycol as precipitant.
Open Access
- 1 May 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 25 (5) , 716-718
- https://doi.org/10.1093/clinchem/25.5.716
Abstract
We devised an enzyme immunoassay for plasma estrogen-like substances in pregnancy, using estriol covalently linked to horseradish peroxidase. Free and bound steroid were separated by use of polyethylene glycol reagent. The assay satisfies the normal criteria of specificity and precision. Results obtained by radioimmunoassay and enzyme immunoassay agreed well. Our assay is particularly applicable in smaller clinical laboratories to routine determination of plasma estriol in pregnancy.This publication has 5 references indexed in Scilit:
- Comparison of double antibody and solid-phase radioimmunoassaysJournal of Immunological Methods, 1978
- A specific enzymeimmunoassay for progesterone in human plasmaSteroids, 1977
- Enzyme-Labelled Immunoassay for Plasma CortisolJournal of Clinical Endocrinology & Metabolism, 1977
- Solid-phase radioimmunoassays of estriol-16α-glucuronide in urine and pregnancy plasmaThe Journal of Steroid Biochemistry and Molecular Biology, 1977
- An immuno-enzymatic assay of cortisol using E. coli β-galactosidase as labelJournal of Immunological Methods, 1976