Mycelial Development and Chemical Alteration ofCandida albicansfrom Biotin Insufficiency

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Abstract
A strain of Candida albicans in a chemically defined basal medium supplemented with biotin at optimal (10 ng/ml) and suboptimal (0.1 ng/ml) concentrations for growth developed predominantly into yeast (Y) and mycelial (M) phases, respectively. The M-phase thus grown showed an increase in alkali-insoluble glucan and a decrease in RNA and mannan as compared with Y- cells grown in biotin-optimal medium. Changes in the cell morphology as well as the chemical composition due to biotin insufficiency were almost completely restored by the supplementation of glyceryl mono-oleate to the medium. However, the supplementation of aspartie acid, accentuated both morphological and biochemical properties which were characteristic for biotin-insufficient cells. The correlation of morphology with the composition of major cellular constituents, especially cell wall polysaceharides, is discussed in relation to the casual role of certain biotin-participating metabolic processes in this fungus.

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