Effect of Cyclic Nucleotides on the Cyanide-insensitive Respiration of Polymorphonuclear Leucocytes 1
- 1 March 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 77 (3) , 567-573
- https://doi.org/10.1093/oxfordjournals.jbchem.a130757
Abstract
The effects of N6, o2'-dibutyryladenosine 3', 5'-monophosphate (Bu2-cyclic AMP) and N2, O2'-dibutyrylguanosine 3', 5'-monophosphate (Bu2-cyclic GMP) on the cyanide-insensitive respiration of guinea pig peritoneal exudate polymorphonuclear leucocytes were studied. Bu2-cyclic AMP inhibited the respiration induced both by phagocytosis of E. coli and by the interaction with trypsin-digested rat liver microsomes. The addition of theophylline gave rise to an inhibitory pattern similar to that with cyclic AMP against both the respirations induced. On the other hand, Bu2-cyclic GMP did not affect the respiration induced by phagocytosis whereas it inhibited the respiration induced by trypsin-digested microsomes. The cyanide-insensitive respiration induced by the addition of myristic acid was inhibited by Bu2-cyclic AMP, which was similar to that with E. coli. The respiration induced by methylene blue was inhibited neither by Bu2-cyclic AMP nor by Bu2-cyclic GMP. These observations suggest that the cyanide-insensitive respiration of polymorphonuclear leucocytes may be classified into at least three types from the inhibitory pattern of cyclic AMP and cyclic GMP.Keywords
This publication has 0 references indexed in Scilit: