Rapid ATP assays in perfused mouse liver by 31P NMR.

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Abstract
31P NMR was used to continuously monitor ATP and Pi levels in perfused mouse liver. Under optimal conditions, the time resolution of the technique was approximately 1 min. In the absence of any metabolic perturbations the ATP level remained constant for at least 2 h and decreased by only approximately 20% in 18 h. Both ATP and Pi levels responded to alterations in the O2 supply to the liver. The half-time for this response was approximately 1 min, and the response to short periods of hypoxia or ischemia was partially reversible. The addition of insulin caused only a minor decrease in the ATP level but significantly decreased the rate of response of ATP and Pi levels to hypoxia and ischemia.