A GENERAL METHOD FOR THE LABELING OF THE ACTIVE SITE OF ANTIBODIES AND ENZYMES

Publisher Website
Google Scholar
Abstract
A general method has been developed for the labeling of the active site of antibodies and enzymes. It consists of 3 steps: (1) the treatment of the protein in the presence of substrate with a specific unlabeled reagent, (2) the removal of the substrate, and (3) the reaction of the groups at the active site with the same reagent in which a radioactive label has been incorporated. The method has successfully incorporated I131 into the active site of rabbit antibody directed against the p-azobenzenearsenate group. The method is of particular practicality because it does not depend on any fortuitous property of the antibody or enzyme and it provides a means for mapping the three-dimensional structure of the active site.