Identification of ColE1 DNA sequences that direct single strand-to-double strand conversion by a phi X174 type mechanism.

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Abstract
A DNA single-strand initiation sequence, named rriA (called rri-1 previously), was detected in the origin region (Hae II fragment E) of the ColE1 plasmid. Another site, called rriB, was found on the opposite strand of Hae II fragment C. Both rriA and rriB direct conversion of chimeric M13 phage single-stranded DNA to parental replicative form DNA in vivo by a rifampicin-resistant mechanism that is dependent on the dnaG and dnaB gene products, provide effector sites of dATP hydrolysis by primosomal protein n'' and require the same primosomal proteins as .vphi.X174 DNA for directing the in vitro conversion of the single-stranded DNA to a double-stranded form. rriA appears to be the DNA sequence that determines the mechanism of lagging strand synthesis of ColE1 DNA and the mechanism of discontinuous synthesis apparently involves the primosomal proteins utilized in the in vitro conversion of .vphi.X174 single strands to the double-stranded replicative form.