The aspartic proteinase from the rodent parasitePlasmodium bergheias a potential model for plasmepsins from the human malaria parasite,Plasmodium falciparum
Open Access
- 7 December 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 463 (1-2) , 43-48
- https://doi.org/10.1016/s0014-5793(99)01597-5
Abstract
The gene encoding an aspartic proteinase precursor (proplasmepsin) from the rodent malaria parasite Plasmodium berghei has been cloned. Recombinant P. berghei plasmepsin hydrolysed a synthetic peptide substrate and this cleavage was prevented by the general aspartic proteinase inhibitor, isovaleryl pepstatin and by Ro40‐4388, a lead compound for the inhibition of plasmepsins from the human malaria parasite Plasmodium falciparum. Southern blotting detected only one proplasmepsin gene in P. berghei. Two plasmepsins have previously been reported in P. falciparum. Here, we describe two further proplasmepsin genes from this species. The suitability of P. berghei as a model for the in vivo evaluation of plasmepsin inhibitors is discussed.Keywords
This publication has 13 references indexed in Scilit:
- On the size of the active site in proteases. I. PapainPublished by Elsevier ,2005
- A distinct member of the aspartic proteinase gene family from the human malaria parasite Plasmodium falciparumFEBS Letters, 1999
- Expression and Characterisation of Plasmepsin I from Plasmodium falciparumEuropean Journal of Biochemistry, 1997
- MAB, a generally applicable molecular force field for structure modelling in medicinal chemistryJournal of Computer-Aided Molecular Design, 1995
- Sequence, expression and modeled structure of an aspartic proteinase from the human malaria parasite Plasmodium falciparumMolecular and Biochemical Parasitology, 1994
- Cloning and characterization of an Eimeria acervulina sporozoite gene homologous to aspartyl proteinasesMolecular and Biochemical Parasitology, 1993
- Crystal structures of native and inhibited forms of human cathepsin D: implications for lysosomal targeting and drug design.Proceedings of the National Academy of Sciences, 1993
- Assessment of protein models with three-dimensional profilesNature, 1992
- Hemoglobin degradation in the human malaria pathogen Plasmodium falciparum: a catabolic pathway initiated by a specific aspartic protease.The Journal of Experimental Medicine, 1991
- Basic local alignment search toolJournal of Molecular Biology, 1990