Enzyme-linked Immunosorbent Assay for Coronaviruses HCV 229E and MHV 3
- 1 July 1980
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 49 (1) , 83-89
- https://doi.org/10.1099/0022-1317-49-1-83
Abstract
The antigenic relationship between human coronavirus strain 229E (HCV 229E) and mouse hepatitis virus strain 3 (MHV 3) was studied by means of the indirect form of enzyme-linked immunosorbent assay (ELISA). A cross-reaction was found with hyperimmune rabbit sera between HCV 229E and MHV 3 which may be due to the adherence of bovine serum components from tissue culture media, which were present on virus particles even after extensive purification. No cross-reaction was observed with immune sera absorbed with bovine serum, or with HCV 229E grown in tissue culture without serum. This indirect ELISA with HCV 229E may prove to be useful for studies with human sera.This publication has 4 references indexed in Scilit:
- The Genome of Human Coronavirus Strain 229EJournal of General Virology, 1978
- Ribonucleoprotein-like Structures from Coronavirus ParticlesJournal of General Virology, 1978
- Antigenic relationship of the feline infectious peritonitis virus to coronaviruses of other speciesArchiv für die gesamte Virusforschung, 1978
- Antibodies to Mouse Hepatitis Viruses in Human SeraExperimental Biology and Medicine, 1964