A new and versatile fluorescence standard for quantum yield determination
- 20 December 2000
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Physical Chemistry Chemical Physics
- Vol. 3 (2) , 172-174
- https://doi.org/10.1039/b007671f
Abstract
It is demonstrated that a new bichromophoric compound, N2,N3-[bis(1-hexylheptyl)-benzo[ghi]perylene-2,3,8,9,11,12-hexacarboxylic-2,3:8,9:11,12-tris(dicarboximide)]-N1,N1′-(1,2-ethyl)-[N2′-(1-octylnonyl)-perylene-3,4:9,10-bis(dicarboximide)] (denoted by 1–2), provides a versatile reference molecule for measurements of fluorescence quantum yields. The compound consists of a donor (1) and an acceptor (2) part for the electronic energy. Absorption spectra of 1–2 form a nearly perfect superposition of the corresponding spectra for 1 and 2. In addition to a fluorescence quantum yield of one, 1–2 allows for choosing excitation wavelengths between 300 and 530 nm. The fluorescence lifetime of 1–2 is 4.0 ± 0.1 ns and is independent of the excitation wavelength. The fluorescence excitation anisotropy reveals that the S0 → S1 and S0 → S2 transition dipoles of 1 are preferentially polarised parallel and perpendicular, respectively, to the C2-symmetry axis of 1. The rapid transfer of electronic energy is compatible with energy transfer of the Förster type.Keywords
This publication has 0 references indexed in Scilit: