Agglutination reactions of spontaneous canine tumour cells, induced by concanavalin a, demonstrated by an isotopic assay

Publisher Website
Google Scholar
Abstract
Quantitative assessment of the agglutination of 51Cr labeled canine cell suspensions to canine kidney cell monolayers was performed over a range of concanavalin A concentrations. Agglutination was observed with all cell cultures tested, comprising 4 spontaneous canine melanomas, 2 canine mammary carcinomas, a benign mammary tumor and a contact inhibited kidney cell line. The melanomas tested showed strong specific inhibition of concanavalin A agglutination by 10-2 M .alpha.-methyl-D-glucopyranoside. Inhibition of agglutination of mammary tumor and kidney cells was weaker and less specific. Agglutination was inhibited at 4.degree. C. Reduced agglutination to glutaraldehyde-fixed monolayers was observed in mammary tumors but was absent when contact inhibited kidney cells were tested. The specificity of the reaction for transformed cells and the parameters involved are discussed.