Deacetyl-thymosin .ALPHA.1: Synthesis and immunological effect on lipoid nephrosis lymphocytes.

Publisher Website
Google Scholar
Abstract
The Ac-Ser residue of bovine thymosin .alpha.1 (position 1) was replaced with Ser to examine the resulting change in immunological effect on the low rosette-forming capacity with sheep erythrocytes of cells from a lipoid nephrosis patient. The deacetyl-thymosin .alpha.1 was synthesized by the solution method. For the synthesis of the protected octaeicosapeptide, 5 peptide fragments were prepared by the stepwise elongation method with N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline as a coupling reagent. The condensations of the fragments were achieved by Rudinger''s azide procedure. All protecting groups were removed by HF treatment followed by catalytic hydrogenation. The in vitro addition of the synthetic deacetyl-thymosin .alpha.1 at a dose of 100 .mu.g/ml was able to restore the rosette-forming capacity with sheep erythrocytes of lipoid nephrosis cells to normal levels. Preparations of deacetyl-thymosin .alpha.1 and thymosin .alpha.1 were equally active in cases of lipoid nephrosis.