CaNAT1 , a Heterologous Dominant Selectable Marker for Transformation of Candida albicans and Other Pathogenic Candida Species
Open Access
- 1 February 2005
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 73 (2) , 1239-1242
- https://doi.org/10.1128/iai.73.2.1239-1242.2005
Abstract
A dominant selectable marker for Candida albicans and other Candida species, which confers resistance to nourseothricin, was characterized. In a heterologous promoter system and a recyclable cassette, the marker efficiently permitted deletion and complementation of C. albicans genes. Neither growth nor filamentous development was affected in strains expressing this marker.Keywords
This publication has 19 references indexed in Scilit:
- Evaluation of the Roles of Four Candida albicans Genes in Virulence by Using Gene Disruption Strains That Express URA3 from the Native LocusInfection and Immunity, 2003
- Large‐scale essential gene identification in Candida albicans and applications to antifungal drug discoveryMolecular Microbiology, 2003
- Development of Streptococcus thermophilus lacZ as a reporter gene for Candida albicansMicrobiology, 2001
- Cassettes for PCR‐mediated construction of green, yellow, and cyan fluorescent protein fusions in Candida albicansYeast, 2001
- Single-Copy IMH3 Allele Is Sufficient To Confer Resistance to Mycophenolic Acid in Candida albicans and To Mediate Transformation of Clinical Candida SpeciesInfection and Immunity, 2001
- Three new dominant drug resistance cassettes for gene disruption inSaccharomyces cerevisiaeYeast, 1999
- Non-universal Usage of the Leucine CUG Codon and the Molecular Phylogeny of the Genus CandidaSystematic and Applied Microbiology, 1999
- Suitability of replacement markers for functional analysis studies inSaccharomyces cerevisiaeYeast, 1997
- New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiaeYeast, 1994
- The codon CUG is read as serine in an asporogenic yeast Candida cylindraceaNature, 1989