Sensor–tissue interactions in neurochemical analysis with carbon paste electrodes in vivo
- 1 January 1993
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 118 (4) , 433-438
- https://doi.org/10.1039/an9931800433
Abstract
Characterization of voltammetric signals recorded with microelectrodes in the living brain is fraught with difficulties. In addition to being anatomically complicated, brain tissue presents the analytical electrochemist with a complex chemical environment that includes surfactants (lipids), electrode poisons (proteins), electrocatalysts such as glutathione and ascorbic acid, and a tissue matrix that both restricts mass transport to the electrode surface and reacts physiologically to the presence of the probe. Identification of electrochemical signals recorded in vivo with carbon paste electrodes is discussed in the context of these problems. This examination shows that modification of both the electrode surface by tissue, and of the tissue environment by the electrode, have important implications for voltammetric signal analysis in vivo. Despite these problems, valuable data on the relationship between behaviour and chemical changes in the brain can be obtained using in vivo electrochemical techniques.Keywords
This publication has 64 references indexed in Scilit:
- Microdialysis—principles and applications for studies in animals and manJournal of Internal Medicine, 1991
- Regional distribution of ascorbate and 3,4-dihydroxyphenylacetic acid (DOPAC) in rat striatumBrain Research, 1991
- General histopathological changes in the central nervous system with ageActa Oto-Laryngologica, 1991
- Stearate-modified carbon paste electrodes for detecting dopamine in vivo: decrease in selectivity caused by lipids and other surface-active agentsAnalytical Chemistry, 1990
- The oxidation of ascorbic acid at carbon paste electrodesJournal of Electroanalytical Chemistry and Interfacial Electrochemistry, 1990
- Brain MicrodialysisJournal of Neurochemistry, 1989
- In vivo voltammetry—Present electrodes and methodsNeuroscience, 1988
- The steepness of voltammetric wavesJournal of Electroanalytical Chemistry and Interfacial Electrochemistry, 1985
- Linear sweep voltammetry with carbon paste electrodes in the rat striatumNeuroscience, 1982
- Theory of Stationary Electrode Polarography. Single Scan and Cyclic Methods Applied to Reversible, Irreversible, and Kinetic Systems.Analytical Chemistry, 1964