High-Pressure Liquid Chromatographic Determination of Vitamin D3 in Resins, Oils, Dry Concentrates, and Dry Concentrates Containing Vitamin A
- 1 March 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 59 (2) , 251-260
- https://doi.org/10.1093/jaoac/59.2.251
Abstract
A high-pressure liquid chromatographic (HPLC) procedure has been developed for the determination of vitamin D3 in resins, oils, dry concentrates, and dry concentrates containing vitamin A. Specificity of the method for vitamin D3 in the presence of isomers and other related constituents was shown by ultimate recovery of the vitamin D3 and the individual constituents and their characterization by other methods such as ultraviolet spectrophotometry. Precision and accuracy are within 2%, and as many as 20 determinations may be completed in a working day. Excellent agreement with the official AOAC biological assay was found. A comparison of the response of isomers of vitamin D3 by the HPLC method vs. other instrumental and chemical procedures shows HPLC to be the most specific method for determining the bioactive isomers.This publication has 2 references indexed in Scilit:
- STUDIES ON THE CHEMICAL ISOMERIZATION OF VITAMIN DTHE JOURNAL OF VITAMINOLOGY, 1967
- STUDIES ON THE CHEMICAL ISOMERIZATION OF VITAMIN DTHE JOURNAL OF VITAMINOLOGY, 1965