Strahlenbedingte Frühveränderungen an Zellkern-Proteinen

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Abstract
The nuclear albumin and histone fractions from cell nuclei of the liver, from whole body irradiated rats (1000 r) were extracted and their nitrogen and nucleic acid contents analyzed. Parallel experiments were run with nuclei of rat liver isolated by the Behrens method and with nuclei of mouse liver isolated with nuclei of mouse liver isolated with sugar. A reduction of nucleoprotein in both fractions beginning immediately after irradiation was found. In vivo irradiation injury of the desoxyribonucleic acid-histone complexes in the liver led to degradation of large amounts of histone without changes in desoxyribonucleic acid (DNA) concentration. The changes induced by irradiation, can be divided into two periods: early changes and, spearated from them by a brief period of recovery, extensive but slower nucleoprotein degradation, which is reversible only in parencyhmatous cell nuclei. In the lymphatic nucleus the loss of nucleoprotein and DNA leads to the destruction of the nucleus. In the irradiation induced loss of histone in the spleen and thymus we see an early stage in the chain of action, which in irradiated lymphocytes leads to the degradation of the DNA-nucleoprotein complex.

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