The human leukocyte test system
- 1 January 1976
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 35 (1) , 57-70
- https://doi.org/10.1007/bf00295619
Abstract
Leukocyte cultures were set up with X-irradiated whole blood (200 R). Cells starting with their DNA synthesis between 25 and 35 h after culture initiation (“early replicating cells”) were pulse-labeled with tritiated thymidine ([3H] TdR). Mitoses were collected with colcemid in adjacent intervals from 36 up to 72 h after culture initiation. At fixation times of 50, 56, 62, and 72 h enough mitoses for a determination of the frequencies of chromosomal aberrations (dicentric and ring chromosomes) were found. After that the preparations were processed for autoradiography. All mitoses analyzed for chromosomal aberrations were re-analyzed for labeling, and the frequencies of chromosomal aberrations in labeled (=“early replicating cells”) and unlabeled (=“late replicating cells”) mitoses were compared. At all fixation times, higher frequencies of dicentric chromosomes were found in labeled as compared to unlabeled mitoses, indicating a higher sensitivity of early replicating cells to X-irradiation in the G0 stage of the cell cycle.Keywords
This publication has 28 references indexed in Scilit:
- Heterogenous chromosomal radiosensitivity of phytohaemagglutinin-stimulated human blood lymphocytes in cultureMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1973
- Biological Damage from Intranuclear Tritium: DNA Strand Breaks and Their RepairScience, 1972
- DNA synthesis and distribution of first and second divisions in 72-h-cultures of human leukocytesCellular and Molecular Life Sciences, 1972
- Studies on in vitro lymphocyte proliferation in cultures synchronized by the inhibition of DNA synthesis: II. Kinetics of the initiation of the proliferative responseExperimental Cell Research, 1970
- Factors influencing chromosome aberration yields in the human peripheral leukocyte systemMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1969
- Studies on in vitro lymphocyte proliferation in cultures synchronized by the inhibition of DNA synthesis: I. Variability of S plus G2 periods of first generation cellsExperimental Cell Research, 1969
- Comparisons of Tritiated Thymidine, Tritiated Water, and Colbalt-60 Gamma Rays in Inducing Chromosomal AberrationsRadiation Research, 1965
- Chromosome Investigations on Lymphocytes from Irradiated Patients: Effect of Time in CultureNature, 1964
- Time-course of DNA synthesis in human leukocyte culturesExperimental Cell Research, 1963
- Aberrations Induced in Human Leukocyte Chromosomes by 3H-Labeled NucleosidesCytogenetic and Genome Research, 1962