Acetylcholine-induced change in intracellular Cl? activity of the mouse lacrimal acinar cells

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Abstract
Using double-barreled Cl−-sensitive microelectrodes, intracellular Cl− activity (A Cl i ) in the mouse lacrimal acinar cells in vitro was determined in both resting and secretory phases. In the resting stateA Cl i was 31 mmol/l which was 1.4 times higher than that predicted for the passive distribution according to the membrane potential (Vm) of −41 mV. Addition of acetylcholine (ACh, 1μM) hyperpolarizedVm to −63 mV and decreasedA Cl i to 20 mmol/l which was still twice the equilibrium activity. A-23178 produced similar changes inVm andA Cl i to those induced by ACh. It was concluded that Cl− was actively accumulated in the acinar cells and, in the secretory phase, Cl− efflux was enhanced by the increased driving force and Ca2+-mediated increase in the Cl− permeability across the cell membrane.