ANTI-IMMUNOGLOBULIN STIMULATION OF MURINE LYMPHOCYTES .1. AGE DEPENDENCY OF PROLIFERATIVE RESPONSE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 116  (6) , 1656-1661
Abstract
The in vitro proliferative response of normal mouse spleen cells to [rabbit] anti-immunoglobulin (Anti-Ig) reagents was an age-associated phenomenon. The response usually appears in mice at 5-7 months of age and is rarely seen in younger animals. Anti-Ig induced proliferation was observed by using 2 different antisera; 1 polyvalent, prepared against mouse antibody-antigen [Salmonella adelaide] complexes and 1 prepared against mouse IgM [immunoglobulin M] myeloma. Both antisera were specific for B [bone marrow-derived] cells by cytotxicity and immunofluorescent staining. Glassbead separation of spleen cells showed that the anti-Ig-induced proliferation was a B cell response.

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