Gene order on the short arm of human chromosome 11: regional assignment of the LDH A gene distal to catalase in two translocations
- 1 November 1985
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 71 (3) , 249-253
- https://doi.org/10.1007/bf00284584
Abstract
Leukemic cells with reciprocal translocations involving 11p13 and 14q13 were obtained from two patients with T-cell acute lymphoblastic leukemia and fused with mouse Ltk- cells. DNA from independent hybrid clones was screened by Southern blot and hybridization to molecular probes for the human catalase and Ha-ras-1 genes. Several clones showed segregation of these two genes, indicating the presence of either the der 11 or der 14 human chromosomes. When DNA from these hybrid clones was examined for the presence of the human genes for calcitonin and γ-globin, both genes were found to segregate with the Ha-ras-1 gene and the der14 chromosome indicating that they lie distal to catalase. When the hybrid clones were examined for the presence of human lactate dehydrogenase A (LDH A) activity, only those clones containing the der14 chromosome expressed activity indicating that the LDH A gene is also distal to catalase on the short arm of chromosome 11.Keywords
This publication has 26 references indexed in Scilit:
- Breakpoints in the human T-cell antigen receptor α-chain locus in two T-cell leukaemia patients with chromosomal translocationsNature, 1985
- Germ-Line chromosomal localization of genes in chromosome 11p linkage: Parathyroid hormone, ?-globin, c-Ha-ras-1, and insulinSomatic Cell and Molecular Genetics, 1985
- New chromosomal translocations correlate with specific immunophenotypes of childhood acute lymphoblastic leukemiaCell, 1984
- Report of the committee on the genetic constitution of chromosomes 10, 11, and 12Cytogenetic and Genome Research, 1984
- c-Ha-ras1 is not deleted in aniridia–Wilms' tumour associationNature, 1983
- The c-Ha-ras1, insulin and β-globin loci map outside the deletion associated with aniridia-Wilms' tumourNature, 1983
- Oncogene from Human EJ Bladder Carcinoma Is Located on the Short Arm of Chromosome 11Science, 1983
- Chromosomal localization of a single copy gene by in situ hybridization ‐ human β globin genes on the short arm of chromosome 11Annals of Human Genetics, 1981
- Gene dose effect: intraband mapping of the LDH A locus using cells from four individuals with different interstitial deletions of 11pCytogenetic and Genome Research, 1977
- Enzyme electrophoresis on cellulose acetate gel: Zymogram patterns in man-mouse and man-Chinese hamster somatic cell hybridsArchives of Biochemistry and Biophysics, 1971