4E Binding Proteins Inhibit the Translation Factor eIF4E without Folded Structure
- 1 January 1998
- journal article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 37 (1) , 9-15
- https://doi.org/10.1021/bi972494r
Abstract
The 4E binding proteins (4E-BP1 and 4E-BP2) inhibit translation by binding to the limiting, proto-oncogenic initiation factor eIF4E. 4E-BPs produced in Escherichia coli had little or no folded structure, measured by NMR and CD. However, these proteins inhibited translation in reticulocyte lysate. Furthermore, they bound to isolated mouse eIF4E, showing a few broader, dispersed new NMR signals but no general increase in chemical shift dispersion. A peptide with the sequence of 4E-BP1 residues 49−68 was sufficient to bind eIF4E and to inhibit translation in reticulocyte lysate. These results suggest that a short central region of the 4E-BPs is responsible for eIF4E binding and translation inhibition while the remainder is unfolded and flexible.Keywords
This publication has 35 references indexed in Scilit:
- The importance of being unfoldedNature, 1997
- Angiotensin II Stimulates Phosphorylation of the Translational Repressor 4E-binding Protein 1 by a Mitogen-activated Protein Kinase-independent MechanismPublished by Elsevier ,1997
- Tissue Distribution, Genomic Structure, and Chromosome Mapping of Mouse and Human Eukaryotic Initiation Factor 4E-Binding Proteins 1 and 2Genomics, 1996
- Control of the Translational Regulators PHAS-I and PHAS-II by Insulin and cAMP in 3T3-L1 AdipocytesPublished by Elsevier ,1996
- Structure of the C-Terminal Region of p21WAF1/CIP1 Complexed with Human PCNACell, 1996
- Phosphorylation of eIF-4E on Serine 209 by Protein Kinase C Is Inhibited by the Translational Repressors, 4E-binding ProteinsJournal of Biological Chemistry, 1996
- Control of PHAS-I by Insulin in 3T3-L1 AdipocytesJournal of Biological Chemistry, 1995
- Relationship between nuclear magnetic resonance chemical shift and protein secondary structureJournal of Molecular Biology, 1991
- High-level synthesis in Escherichia coli of functional cap-binding eukaryotic initiation factor eIF-4E and affinity purification using a simplified cap-analog resinGene, 1988
- Continuous protein synthesis is required to maintain the probability of entry into S phaseCell, 1977