Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae.
- 1 December 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (24) , 8839-8843
- https://doi.org/10.1073/pnas.84.24.8839
Abstract
A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoter. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. .alpha.-Amanitin (10 .mu.g/ml) and chloride (100 mM) were highly inhibitory.This publication has 20 references indexed in Scilit:
- The relationship between the "TATA" sequence and transcription initiation sites at the HIS4 gene of Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences, 1985
- Accurate transcription of cloned Neurospora RNA polymerase II-dependent genes in vitro by homologous soluble extracts.Proceedings of the National Academy of Sciences, 1985
- Specific protein binding to far upstream activating sequences in polymerase II promoters.Proceedings of the National Academy of Sciences, 1985
- Complex regulation of simian virus 40 early-region transcription from different overlapping promoters.Molecular and Cellular Biology, 1984
- Yeast promoters: Positive and negative elementsCell, 1984
- A Drosophila RNA polymerase II transcription factor contains a promoter-region-specific DNA-binding activityCell, 1984
- Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoterNucleic Acids Research, 1984
- RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.Proceedings of the National Academy of Sciences, 1983
- The isolation, characterization, and sequence of the pyruvate kinase gene of Saccharomyces cerevisiae.Journal of Biological Chemistry, 1983
- The effect of volume occupancy upon the thermodynamic activity of proteins: some biochemical consequencesMolecular and Cellular Biochemistry, 1983