Limited proteolysis of the porcine zona pellucida by homologous sperm acrosin
- 1 May 1985
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 74 (1) , 257-260
- https://doi.org/10.1530/jrf.0.0740257
Abstract
Boar sperm acrosin isolated by affinity chromatography on p-(p''-aminophenoxypropoxy)benzamidine linked to Sepharose was tested for its proteolytic effect on the zona pellucida of freshly ovulated pig eggs. During 1 h in a physiological medium there was no observable change in the morphology of the zona pellucida but subsequent 125I labeling of the structure followed by electrophoretic analysis revealed that acrosin had asserted a limited and selective proteolysis.This publication has 10 references indexed in Scilit:
- Ultrastructural localization of proacrosin and acrosin in ram spermatozoaGamete Research, 1984
- Purification of mouse sperm acrosin, its activation from proacrosin and effect on homologous egg investmentsReproduction, 1983
- Involvement of trypsin-like activity in binding of mouse spermatozoa to zonae pellucidae.Proceedings of the National Academy of Sciences, 1981
- Isolation, physicochemical properties, and macromolecular composition of zona pellucida from porcine oocytesBiochemistry, 1980
- Normal penetration of rabbit spermatozoa through a trypsin- and acrosin-resistant zona pellucidaReproduction, 1978
- Studies on ram acrosin. Activation of proacrosin accompanying the isolation of acrosin from spermatozoa, and purification of the enzyme by affinity chromatographyBiochemical Journal, 1978
- The activation of proteolysis in the acrosome reaction of guinea-pig spermJournal of Cell Science, 1978
- BOAR M-ALPHA-ACROSIN - PURIFICATION AND CHARACTERIZATION OF INITIAL ACTIVE ENZYME RESULTING FROM CONVERSION OF BOAR PROACROSIN TO ACROSIN1978
- Effects of Acrosin Inhibitors on the Soluble and Membrane-Bound Forms of Ram Acrosin, and a Reappraisal of the Role of the Enzyme in FertilizationHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1976
- PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSESThe Journal of Experimental Medicine, 1954