Characterization of Amino Acid Substitutions in KdpA, the K + -Binding and -Translocating Subunit of the KdpFABC Complex of Escherichia coli
- 1 October 2002
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 184 (19) , 5491-4
- https://doi.org/10.1128/jb.184.19.5491-5494.2002
Abstract
When grown under K + limitation, Escherichia coli induces the K + -translocating KdpFABC complex. The stimulation of ATPase activity by NH 4 + ions was shown for the first time. Substitutions in KdpA, which is responsible for K + binding and translocation, revealed that enzyme complexes KdpA:G232A and KdpA:G232S have completely lost their cation selectivity.Keywords
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