Analysis of Phosphoramide Mustard by Reversed-Phase Ion Pair High Pressure Liquid Chromatography
- 1 June 1987
- journal article
- research article
- Published by Wolters Kluwer Health in Therapeutic Drug Monitoring
- Vol. 9 (2) , 221-226
- https://doi.org/10.1097/00007691-198706000-00017
Abstract
The analysis of phosphoramide mustard by reversed-phase ion pair high pressure liquid chromatography using ultraviolet detection is described. The method will enable measurement of serum concentrations of phosphoramide mustard over a period of 24 h after an i.v. dose of 60 mg/kg of cyclophosphamide and requires 0.5 ml of serum or plasma. The between-day precision of the assay at concentrations of 0.34, 2, 76, and 6, 90 mg/L gave coefficients of variation of 16, 10, and 9%, respectively. The percentage analytical recovery of phosphoramide mustard is 35%. Preliminary results providing pharmacokinetic data on two adults undergoing high-close cyclophosphamide treatment prior to bone marrow transplant are presented.Keywords
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