Determination of Phenytoin in Plasma: A Comparison of Procedures Using a New Radioimmunoassay, Gas Chromatography, and Enzyme Immunoassay
- 1 June 1978
- Vol. 19 (3) , 265-272
- https://doi.org/10.1111/j.1528-1157.1978.tb04489.x
Abstract
A new commercially available radioimmunoassay (RIA) procedure for the anticonvulsant drug phenytoin was assessed and compared with a GLC and an enzyme immunoassay (EMIT) procedure. Human plasma samples (134) containing phenytoin were analyzed by the 3 methods and the results were found to be essentially independent of the procedure used and so would indicate similar clinical interpretation. The correlation coefficients were: RIA/GLC = 0.988; EMIT/RIA = 0.978; EMIT/GLC = 0.981. The cross reactivity of 33 drugs and related compounds was tested in the RIA and EMIT procedures and only a few very closely related substances showed significant cross reactivity. The 3 methods were compared with respect to the technical difficulties involved as well as time and cost for the assay of single and multiple samples.Keywords
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