Redox levels of intravenously administered (14C)coenzyme Q10 and coenzyme Q10-reducing activity in subcellular fractions of guinea pig liver.

Abstract
The blood level of [14C]coenzyme Q10 and the redox levels of [14C]coenzyme Q10 in the liver and heart were measured after i.v. injection of [14C]coenzyme Q10 solubilized in multilamellar liposomes into guinea pigs. The blood level of radioactivity declined biexponentially with 1/2-lives of 11.5 min and 15.6 h in the 1st and 2nd phases, respectively. The levels of reduced [14C]coenzyme Q10 in the liver and heart reached 55.8 and 46.4%, respectively, of the labeled compound in the tissues at 30 min after the injection. Coenzyme Q10 reducing activity in cytosol, microsomes and mitochondria was also investigated. This activity was found in all fractions. The total activity was the highest in the liver cytosol. The results of experiments using a purified enzyme suggested that 1 of the coenzyme Q10-reducing enzymes was NAD(P)H:quinone oxidoreductase [EC 1.6.99.2, DT-diaphorase]. Results are discussed in relation to the protective effect of reduced coenzyme Q10 against lipid peroxidation in membranes.