Fluorescence Time‐Resolved Spectroscopy and Fluorescence Anisotropy Decay of the Staphylococcus aureus Endonuclease
- 1 February 1974
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 41 (3) , 577-583
- https://doi.org/10.1111/j.1432-1033.1974.tb03299.x
Abstract
The intrinsic fluorescence of Staphylococcus aureus endonuclease has been studied by the time‐resolved spectroscopy method. The tyrosine emission, with a decay time of 1.8 ns, is separated from the tryptophan emission with a decay time of 5.5 ns.The binding of an inhibitor, thymidine 3′,5′‐bisphosphate (pdTp), decreases the intensity of the tyrosine spectrum.The molecular correlation time determined by the anisotropy decay measurement of the tryptophanyl residue is 8.9 ns. This value corresponds to a strongly hydrated molecule.The fixation of pdTp does not change this correlation time, showing that no gross structural modifications are induced by the inhibitor.This publication has 18 references indexed in Scilit:
- Résolution des spectres de fluorescence au moyen des déclinsBiochimica et Biophysica Acta (BBA) - Protein Structure, 1972
- Mesures des declins de l'anisotropie de fluorescence de la gamma-globuline et de ses fragments Fab, Fc et F(ab)2 marques avec le l-sulfonyl-5-dimethyl-aminonaphtaleneEuropean Journal of Biochemistry, 1972
- Segmental flexibility in an antibody moleculeJournal of Molecular Biology, 1970
- The Analysis of Fluorescence Decay by a Method of MomentsBiophysical Journal, 1969
- Mesure de la décroissance de la fluorescence polarisée de la γ-globuline-1-sulfonyl-5-diméthylaminonaphtalèneBiochimica et Biophysica Acta (BBA) - Protein Structure, 1969
- Rendements quantiques de fluorescence et durées de vie radiative de quelques solutés utilisés dans les scintillateurs liquidesJournal de Physique et le Radium, 1966
- Analyse par echantillonnage sur photons individuels des liquides fluorescents dans le domaine de la sub-nanosecondeNuclear Instruments and Methods, 1964
- Enumeration of Components in Complex Systems by Fluorescence SpectrophotometryNature, 1961
- Mouvement Brownien d'un ellipsoide (II). Rotation libre et dépolarisation des fluorescences. Translation et diffusion de molécules ellipsoidalesJournal de Physique et le Radium, 1936
- Mouvement brownien d'un ellipsoide - I. Dispersion diélectrique pour des molécules ellipsoidalesJournal de Physique et le Radium, 1934