Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes

24 November 1988

journal article
research article
Published by Springer Nature in Nature

Vol. 336 (6197) , 348-352
https://doi.org/10.1038/336348a0

Abstract

Gene targeting—homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences—in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive and negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt⁻ and int-2⁻ mutants, but it should be applicable to any gene

Keywords

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