Rapid and Complete Purification of Acetylcholinesterases of Electric Eel and Erythrocyte by Affinity Chromatography
- 1 February 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (2) , 395-398
- https://doi.org/10.1073/pnas.68.2.395
Abstract
Affinity chromatography has been used to purify acetylcholinesterase both from the electric tissue of Electrophorus electricus and from bovine erythrocyte membranes. For this purpose, several specific enzymic inhibitors of each protein were synthesized and joined covalently to an insoluble support resin. AchE is selectively retained by such inhibitor-resins when highly impure solutions are chromatographed upon them. After removal from the resin, both enzymes are electrophoretically homogeneous and they may be recovered in yields of 75% or more.Keywords
This publication has 9 references indexed in Scilit:
- Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.1970
- The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel ElectrophoresisJournal of Biological Chemistry, 1969
- Selective enzyme purification by affinity chromatography.Proceedings of the National Academy of Sciences, 1968
- Affinity labeling of the acetylcholine-receptor.Proceedings of the National Academy of Sciences, 1967
- The Preparation and Properties of Some Tritiated Diazonium Salts and Related Compounds*Biochemistry, 1967
- ACETYLCHOLINESTERASE, I. LARGE-SCALE PURIFICATION, HOMOGENEITY, AND AMINO ACID ANALYSISProceedings of the National Academy of Sciences, 1967
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- Purification of cholinesterase from ox red cellsBiochimica et Biophysica Acta, 1955
- Purification of human red cell acetylcholinesterase by electrophoresis, ultracentrifugation and gradient extractionArchives of Biochemistry and Biophysics, 1955