The Explosive Human Immunodeficiency Virus Type 1 Epidemic among Injecting Drug Users of Kathmandu, Nepal, Is Caused by a Subtype C Virus of Restricted Genetic Diversity
Open Access
- 1 February 2000
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 74 (3) , 1149-1157
- https://doi.org/10.1128/jvi.74.3.1149-1157.2000
Abstract
An explosive epidemic of human immunodeficiency virus type 1 (HIV-1) has been documented among the injecting drug user population of Kathmandu, Nepal, whose seropositivity rate has risen from 0 to 40% between 1995 and 1997. By using Catrimox to preserve whole-blood RNA at ambient temperature for transportation, HIV-1 envelope V3–V4 sequences were obtained from 36 patients in this group. Analysis of the sequences indicated a homogenous epidemic of subtype C virus, with at least two independent introductions of the virus into the population. Viral diversity was restricted within two transmission subclusters, with the majority of variation occurring in V4. Calculation of the synonymous-to-nonsynonymous mutation ratio (Ks:Ka) across this region showed that significant evolutionary pressure had been experienced during the rapid horizontal spread of the virus in this population, most strongly directed to the region between V3 and V4.Keywords
This publication has 22 references indexed in Scilit:
- A Predominantly HIV Type 1 Subtype C-Restricted Epidemic in South African Urban PopulationsAIDS Research and Human Retroviruses, 1999
- Sequence Note: Genomic Sequence of HIV Type 1 from Four Members of the Sydney Blood Bank Cohort of Long-Term NonprogressorsAIDS Research and Human Retroviruses, 1998
- Genetic Characterization of HIV Type 1 from Migrant Workers in Three South African Gold MinesAIDS Research and Human Retroviruses, 1998
- Why do HIV-1 subtypes segregate among persons with different risk behaviors in South Africa and Thailand?AIDS, 1997
- Detection of HIV Type 1envSubtypes A, B, C, and E in Asia Using Dried Blood Spots: A New Surveillance Tool for Molecular EpidemiologyAIDS Research and Human Retroviruses, 1996
- Sequence Note: Envelope Glycoprotein 120 Sequences of Primary HIV Type 1 Isolates from Pune and New Delhi, IndiaAIDS Research and Human Retroviruses, 1996
- Short Communication:envGene Sequences of Primary HIV Type 1 Isolates of Subtypes B, C, D, E, and F Obtained from the World Health Organization Network for HIV Isolation and CharacterizationAIDS Research and Human Retroviruses, 1996
- Genomic Structure of an Attenuated Quasi Species of HIV-1 from a Blood Transfusion Donor and RecipientsScience, 1995
- Direct detection of hepatitis C virus (HCV) RNA from whole blood, and comparison with HCV RNA in plasma and peripheral blood mononuclear cellsJournal of Medical Virology, 1995
- Declining risk for HIV among injecting drug users in Kathmandu, NepalAIDS, 1995