Comparison of 15N-Labelled Glycine, Aspartate, Valine and Leucine for Measurement of Whole-Body Protein Turnover
- 1 September 1979
- journal article
- research article
- Published by Portland Press Ltd. in Clinical Science
- Vol. 57 (3) , 281-283
- https://doi.org/10.1042/cs0570281
Abstract
1. Whole-body protein turnover was measured in rats by constant infusion of 15N-labelled glycine, aspartate, valine and leucine and measuring the enrichment of hepatic and renal urea and ammonia nitrogen. 2. The values obtained with [15N]glycine were comparable with values reported with methods based on different assumptions. 3. [15N]Aspartate gave rise to an increased enrichment of urea and ammonia and hence to lower protein-turnover rates. 4. [15N]Valine and [15N]leucine gave low enrichments of nitrogenous end products and hence to high protein-turnover rates. 5. All 15N-labelled amino acids are not equally suitable for measuring whole-body protein turnover by the end-product method. The relative amounts of 15N going to the end products can be predicted from the known individual metabolism of aspartate and the branched-chain amino acids.Keywords
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