Methylotrophic yeasts as factories for the production of foreign proteins
- 1 November 1995
- Vol. 11 (14) , 1331-1344
- https://doi.org/10.1002/yea.320111402
Abstract
In this contribution we discuss the potential of methylotrophic yeasts as hosts for the high level production of valuable foreign proteins. Recent relevant achievements on the intracellular production or secretion of proteins are summarized. Special attention is paid to a specific advantage of the use of methylotrophic yeasts, namely the possibility of accumulating the foreign gene products inside peroxisomes. This approach may be of major advantage when the protein product is toxic for the host cell and, also, to protect these proteins these proteins from undesired side‐effects such as proteolysis or aggregation.Keywords
This publication has 102 references indexed in Scilit:
- The N‐terminus of amine oxidase of Hansenula polymorpha contains a peroxisomal targeting signalFEBS Letters, 1995
- Cloning and characterization of PAS5: a gene required for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris.The Journal of cell biology, 1993
- Chromosomal targeting of replicating plasmids in the yeast Hansenula polymorphaJournal of General Microbiology, 1992
- High level expression, purification, and characterization of the Kunitz-type protease inhibitor domain of protease nexin-2/amyloid β-protein precursorBiochemical and Biophysical Research Communications, 1992
- Targeting efficiencies of various permutations of the consensus C‐terminal tripeptide peroxisomal targeting signalFEBS Letters, 1992
- Targeting signal of the peroxisomal catalase in the methylotrophic yeastHansenula polymorphaFEBS Letters, 1992
- Amino-terminal presequence of the precursor of peroxisomal 3-ketoacyl-CoA thiolase is a cleavable signal peptide for peroxisomal targetingBiochemical and Biophysical Research Communications, 1991
- An impaired peroxisomal targeting sequence leading to an unusual bicompartmental distribution of cytosolic epoxide hydrolaseFEBS Letters, 1991
- PAS3, a Saccharomyces cerevisiae gene encoding a peroxisomal integral membrane protein essential for peroxisome biogenesis.The Journal of cell biology, 1991
- Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copiesGene, 1991