Production of β-Lactamase by Non- Streptomyces Actinomycetales

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Abstract
Supernatants and whole cells from fermentation broths of Micromonospora, Nocardia, Oerskovia , and other genera of the Actinomycetales were examined for the presence of β-lactamase activity by using the chromogenic cephalosporin 87/312. Nearly 60% of the 250 isolates examined produced detectable levels of β-lactamase. All enzyme preparations were active over a range of pH values from 6.5 to 8.2, with maximum activity occurring between 7.0 and 7.8. The preparations varied in their stability at 60°C. An examination of selected enzyme preparations revealed a similarity between substrate specificities of the non- Streptomyces Actinomycetales and gram-negative-bacterial β-lactamases.
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